The Gadsden Flag features a rattlesnake coiled on a yellow area with the phrase "Don't Tread on Me

https://telegra.ph/George-Washingtons-Example-Duty-Unity-and-the-American-Experiment-05-03

The Gadsden Flag services a rattlesnake coiled on a yellow subject with the word "Don't Tread on Me." It originated in colonial America as a image of resistance against British oppression throughout the American Revolution.